In a gram stain what is the purpose of the iodine mordant? Is it to trap the crystal violet in the gram positive bacteria?
What does the Iodine mordant do in a gram stain?
By definition a mordant is a substance that set dyes. It doesn't really contribute to the coloring of the bacteria. The iodine in Gram stain fixes the crystal violet to the bacterial cell wall of the Gram positive bacteria.
Reply:It allows the stain to stick to the bacteria.
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